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SARS-CoV-2 multi-antigen serology assay.

Mazhari R, Ruybal-Pesántez S, Angrisano F, Kiernan-Walker N, Hyslop S, Longley RJ, Bourke C, Chen C, Williamson DA, Robinson LJ, Mueller I, Eriksson EM

  • Journal Methods and Protocols

  • Published 09 Oct 2021

  • Volume 4

  • ISSUE 4

  • Pagination 72

  • DOI


Serology tests are extremely useful for assessing whether a person has been infected with a pathogen. Since the onset of the COVID-19 pandemic, measurement of anti-SARS-CoV-2-specific antibodies has been considered an essential tool in identifying seropositive individuals and thereby understanding the extent of transmission in communities. The Luminex system is a bead-based technology that has the capacity to assess multiple antigens simultaneously using very low sample volumes and is ideal for high-throughput studies. We have adapted this technology to develop a COVID-19 multi-antigen serological assay. This protocol described here carefully outlines recommended steps to optimize and establish this method for COVID-19-specific antibody measurement in plasma and in saliva. However, the protocol can easily be customized and thus the assay is broadly applicable to measure antibodies to other pathogens.