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The dengue virus M protein localises to the endoplasmic reticulum and forms oligomers.

Wong SS, Haqshenas G, Gowans EJ, Mackenzie J

  • Journal FEBS letters

  • Published 08 Mar 2012

  • Volume 586

  • ISSUE 7

  • Pagination 1032-7

  • DOI 10.1016/j.febslet.2012.02.047


The dengue virus membrane (M) protein is a key component of the mature virion. Here, we characterised the cellular behaviour of M using a recombinant protein construct to understand its inherent properties. Using confocal microscopy, we showed that M and its intracellular precursor, prM, localised to the endoplasmic reticulum. M protein was also detected on the cell surface and secreted, suggesting that M can enter the secretory pathway. In addition, cross-linking studies showed that M can form dimers and tetramers. These findings suggest that M behaves as a secretory protein analogous to the major envelope protein E.