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Improved production and purification of interferon-alpha-4a using a T7 RNA polymerase expression system.

Waine GJ, Wines BD, Wang L, McMullen GL

  • Journal Biochemistry international

  • Published 07 Jan 1993

  • Volume 28

  • ISSUE 2

  • Pagination 255-63


An improved method for the synthesis and purification of human interferon-alpha-4a is presented. Interferon-alpha-4a was prepared using a T7 RNA polymerase expression system, where it was expressed from the vector pET-3a. Biologically active interferon-alpha-4a was isolated from the E. coli cells and purified using protamine sulphate precipitation, anion-exchange chromatography and affinity chromatography utilising a monoclonal antibody specific for human interferons-alpha.