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Cells mediating graft rejection in the mouse. III. Ly-1+ precursor T cells generate skin graft rejection.

Loveland BE, McKenzie IF

  • Journal Transplantation

  • Published 21 Jun 1982

  • Volume 33

  • ISSUE 4

  • Pagination 407-10

  • DOI 10.1097/00007890-198204000-00013


Skin graft rejection in ATXBM CBA mice reconstituted with naive (nonsensitized) cells was shown to be mediated predominantly by Ly-1+2- effector T cells. Thus, after the treatment of the inoculum with the monoclonal anti-Ly-1.1 antibody and complement, C57BL/6 skin grafts survived indefinitely, whereas Ly-2 antibody depletion merely delayed the onset of rejection. This showed that a Ly-1+2- precursor cell rather than a Ly-1+2+ cell was the progenitor of the Ly-1+2- graft rejection effector cell. Nevertheless, another T cell subset augmented the rejection of ski grafts and it was concluded that LY-1,2,3+ cells also provided a pool of precursor cells. Thus, it appeared that both Ly-1+ and Ly-1,2,3+ cells can function as precursor cells for the effector cells, which, as shown previously, have the ly-1+ phenotype.