Positions

Co-lab head of the Gilson/Crabb laboratory

Awards

• 2012 Gust-McKenzie medallist, Burnet Institute

Projects (3)

Current (3)

• Host red blood cell modification sustains the virulence of malaria parasites
  Understanding how malaria parasites modify their human host cells to inform new antimalarials.
• Mechanism of antimalarial drug action
  Understanding how blood-stage replication of Plasmodium could be targeted by vaccines and novel drugs.
• Paralysing malaria parasites
  By understanding how parasites gain entry to human cells we can develop drugs that block and then paralyse the parasites so they can do no more harm.

Publications (64)

2017 (5)

• The exported chaperone Hsp70-x supports virulence functions for Plasmodium falciparum blood stage parasites.
  Charnaud SC, Dixon MWA, Nie CQ, Chappell L, Sanders PR, Nebl T, Hanssen E, Berriman M, Chan JA, Blanch AJ, Beeson JG, Rayner JC, Przyborski JM, Tilley L, Crabb BS, Gilson PR
  PLoS One. 2017 Jul; 12(7):e0181656
• An exported protein-interacting complex involved in the trafficking of virulence determinants in Plasmodium-infected erythrocytes.
  Batinovic S, McHugh E, Chisholm SA, Matthews K, Liu B, Dumont L, Charnaud SC, Schneider MP, Gilson PR, de Koning-Ward TF, Dixon MWA, Tilley L
  Nat Commun. 2017 Jul; 8:16044
• Plasmodium falciparum parasites deploy RhopH2 into the host erythrocyte to obtain nutrients, grow and replicate.
  Counihan N, Chisholm SA, Bullen HE, Srivastava A, Sanders PR, Jonsdottir TK, Weiss GE, Ghosh S, Crabb BS, Creek DJ, Gilson PR, de Koning-Ward TF
  eLife. 2017 Mar; 6(pii):e23217
• Host cell remodeling in malaria parasites: a new pool of potential drug targets.
  Gilson PR, Chisholm SA, Crabb BS, de Koning-Ward TF
  Int J Parasitol. 2017 Feb; 47(2-3):119-127
• Optimization of 2-anilino 4-amino substituted quinazolines into potent antimalarial agents with oral in vivo activity.
  Gilson PR, Tan C, Jarman KE, Lowes KN, Curtis JM, Nguyen W, Di Rago AE, Bullen HE, Prinz B, Duffy S, Baell JB, Hutton CA, Jousset Sabroux H, Crabb BS, Avery VM, Cowman AF, Sleebs BE
  J Med Chem. 2017 Jan; 60(3):1171-1188

2016 (13)

• Disrupting the allosteric interaction between the Plasmodium falciparum cAMP-dependent kinase and its regulatory subunit.
  Littler DR, Bullen HE, Harvey KL, Beddoe T, Crabb BS, Rossjohn J, Gilson PR
  J Biol Chem. 2016 Dec; 291(49):25375-25386
• Molecular genetic approaches to malaria research. (Book Chapter)
  Elsworth B, Azevedo MF, Crabb BS, Gilson PR
  in Advances in Malaria Research (Eds. Gaur D, Chitnis CE and Chauhan VS) Wiley Blackwell. 2016 Dec
• Identification of inhibitors that dually target the new permeability pathway and dihydroorotate dehydrogenase in the blood stage of Plasmodium falciparum.
  Dickerman BK, Elsworth B, Cobbold SA, Nie CQ, McConville MJ, Crabb BS, Gilson PR
  Sci Rep. 2016 Nov; 6:37502
• Trafficking of the exported P. falciparum chaperone PfHsp70x.
  Rhiel M, Bittl V, Tribensky A, Charnaud SC, Strecker M, Müller S, Lanzer M, Sanchez C, Schaeffer-Reiss C, Westermann B, Crabb BS, Gilson PR, Külzer S, Przyborski JM
  Sci Rep. 2016 Nov; 6:36174
• Metabolomics-based screening of the Malaria Box reveals both novel and established mechanisms of action.
  Creek DJ, Chua HH, Cobbold SA, Nijagal B, Macrae JI, Dickerman BK, Gilson PR, Ralph SA, McConville MJ
  Antimicrob Agents Chemother. 2016 Oct; 60(11):6650-6663
• Hierarchical phosphorylation of apical membrane antigen 1 is required for efficient red blood cell invasion by malaria parasites.
  Prinz B, Harvey KL, Wilcke L, Ruch U, Engelberg K, Biller L, Lucet I, Erkelenz S, Heincke D, Spielmann T, Doerig C, Kunick C, Crabb BS, Gilson PR, Gilberger TW
  Sci Rep. 2016 Oct; 6:34479
• Plasmodium species: master renovators of their host cells.
  de Koning-Ward TF, Dixon MW, Tilley L, Gilson PR
  Nat Rev Microbiol. 2016 Jul; 14(8):494-507
• Antimalarial activity of novel 4-cyano-3-methylisoquinoline inhibitors against Plasmodium falciparum: design, synthesis and biological evaluation.
  Buskes MJ, Harvey KL, Richards BJ, Kalhor R, Christoff RM, Gardhi CK, Littler DR, Cope ED, Prinz B, Weiss GE, O'Brien NJ, Crabb BS, Deady LW, Gilson PR, Abbott BM
  Org Biomol Chem. 2016 Apr; 14(20):4617-4639
• Proteomic analysis reveals novel proteins associated with the Plasmodium protein exporter PTEX and a loss of complex stability upon truncation of the core PTEX component, PTEX150.
  Elsworth B, Sanders PR, Nebl T, Batinovic S, Kalanon M, Nie CQ, Charnaud SC, Bullen HE, de Koning Ward TF, Tilley L, Crabb BS, Gilson PR
  Cell Microbiol. 2016 Mar; 18(11):1551-1569
• Exploration of 3-methylisoquinoline-4-carbonitriles as protein kinase A inhibitors of Plasmodium falciparum.
  Buskes MJ, Harvey KL, Prinz B, Crabb BS, Gilson PR, Wilson DJ, Abbott BM
  Bioorg Med Chem. 2016 Mar; 24(11):2389-2396
• Maternal-foetal transfer of Plasmodium falciparum and Plasmodium vivax antibodies in a low transmission setting.
  Charnaud SC, McGready R, Herten-Crabb A, Powell R, Guy A, Langer C, Richards JS, Gilson PR, Chotivanich K, Tsuboi T, Narum DL, Pimanpanarak M, Simpson JA, Beeson JG, Nosten F, Fowkes FJ
  Sci Rep. 2016 Feb; 6:20859
• Recruitment of Factor H as a Novel Complement Evasion Strategy for Blood-Stage Plasmodium falciparum Infection.
  Kennedy AT, Schmidt CQ, Thompson JK, Weiss GE, Taechalertpaisarn T, Gilson PR, Barlow PN, Crabb BS, Cowman AF, Tham WH
  J Immunol. 2016 Feb; 196(3):1239-1248
• Overlaying Molecular and Temporal Aspects of Malaria Parasite Invasion.
  Weiss GE, Crabb BS, Gilson PR
  Trends Parasitol. 2016 Jan; 32(4):284-295

2015 (5)

• Macrolides rapidly inhibit red blood cell invasion by the human malaria parasite, Plasmodium falciparum.
  Wilson DW, Goodman CD, Sleebs BE, Weiss GE, de Jong NW, Angrisano F, Langer C, Baum J, Crabb BS, Gilson PR, McFadden GI, Beeson JG
  BMC Biol. 2015 Jul; 13(1):52
• A Plasmodium Falciparum Bromodomain Protein Regulates Invasion Gene Expression.
  Josling GA, Petter M, Oehring SC, Gupta AP, Dietz O, Wilson DW, Schubert T, Längst G, Gilson PR, Crabb BS, Moes S, Jenoe P, Lim SW, Brown GV, Bozdech Z, Voss TS, Duffy MF
  Cell Host Microbe. 2015 Jun; 17(6):741-751
• Advances in molecular genetic systems in malaria.
  de Koning-Ward TF, Gilson PR, Crabb BS
  Nat Rev Microbiol. 2015 May; 13(6):373-387
• Revealing the Sequence and Resulting Cellular Morphology of Receptor-Ligand Interactions during Plasmodium falciparum Invasion of Erythrocytes.
  Weiss GE, Gilson PR, Taechalertpaisarn T, Tham WH, de Jong NW, Harvey KL, Fowkes FJ, Barlow PN, Rayner JC, Wright GJ, Cowman AF, Crabb BS
  PLoS Pathog. 2015 Feb; 11(2):e1004670
• Identification of Potent Phosphodiesterase Inhibitors that Demonstrate Cyclic Nucleotide-Dependent Functions in Apicomplexan Parasites.
  Howard BL, Harvey KL, Stewart R, Azevedo MF, Crabb BS, Jennings IG, Sanders PR, Manallack DT, Thompson PE, Tonkin CJ, Gilson PR
  ACS Chem Biol. 2015 Jan; 10(4):1145-1154

2014 (6)

• Plasmodium falciparum Transfected with Ultra Bright NanoLuc Luciferase Offers High Sensitivity Detection for the Screening of Growth and Cellular Trafficking Inhibitors.
  Azevedo MF, Nie CQ, Elsworth B, Charnaud SC, Sanders PR, Crabb BS, Gilson PR
  PLoS One. 2014 Nov; 9(11):e112571
• Insights and controversies into the role of the key Apicomplexan invasion ligand, Apical Membrane Antigen 1.
  Harvey KL, Yap A, Gilson PR, Cowman AF, Crabb BS
  Int J Parasitol. 2014 Aug; 44(12):853-857
• PTEX is an essential nexus for protein export in malaria parasites.
  Elsworth B, Matthews K, Nie CQ, Kalanon M, Charnaud SC, Sanders PR, Chisholm SA, Counihan NA, Shaw PJ, Pino P, Chan J-A, Azevedo MF, Rogerson SJ, Beeson JG, Crabb BS, Gilson PR, de Koning-Ward TF
  Nature. 2014 Jul; 511(7511):587-591
• The role of parasite Heat Shock Proteins in protein trafficking and host cell remodeling. (Book Chapter)
  Charnaud SC, Crabb BS, Gilson PR
  in Heat Shock Proteins of Malaria (Eds Addmore Shonhai, Gregory L. Blatch). 2014 Mar; Chapter 6:99-118
• Conditional expression of apical membrane antigen 1 in Plasmodium falciparum shows it is required for erythrocyte invasion by merozoites.
  Yap A, Azevedo MF, Gilson PR, Weiss GE, O Neill MT, Wilson DW, Crabb BS, Cowman AF
  Cell Microbiol. 2014 Feb; 16(5):642-656
• Protein export in malaria parasites: an update.
  Elsworth B, Crabb BS, Gilson PR
  Cell Microbiol. 2014 Jan; 16(3):355-363

2013 (5)

• The Plasmodium translocon of exported proteins (PTEX) component thioredoxin-2 is important for maintaining normal blood-stage growth.
  Matthews K, Kalanon M, Chisholm SA, Sturm A, Goodman CD, Dixon MW, Sanders PR, Nebl T, Fraser F, Haase S, McFadden GI, Gilson PR, Crabb BS, de Koning-Ward TF
  Mol Microbiol. 2013 Jul; 89(6):1167-1186
• Identification and prioritization of merozoite antigens as targets of protective human immunity to Plasmodium falciparum malaria for vaccine and biomarker development.
  Richards JS, Arumugam TU, Reiling L, Healer J, Hodder AN, Fowkes FJ, Cross N, Langer C, Takeo S, Uboldi AD, Thompson JK, Gilson PR, Coppel RL, Siba PM, King CL, Torii M, Chitnis CE, Narum DL, Mueller I, Crabb BS, Cowman AF, Tsuboi T, Beeson JG
  J Immunol. 2013 Jun; 191(2):795-809
• Inhibition of Plasmodium falciparum CDPK1 by conditional expression of its J-domain demonstrates a key role in schizont development.
  Azevedo MF, Sanders PR, Krejany E, Nie CQ, Fu P, Bach LA, Wunderlich G, Crabb BS, Gilson PR
  Biochem J. 2013 Apr; 452(3):433-441
• Spatial association with PTEX complexes defines regions for effector export into Plasmodium falciparum-infected erythrocytes.
  Riglar DT, Rogers KL, Hanssen E, Turnbull L, Bullen HE, Charnaud SC, Przyborski J, Gilson PR, Whitchurch CB, Crabb BS, Baum J, Cowman AF
  Nat Commun. 2013 Jan; 4:1415
• Host cell remodelling and protein trafficking.
  Haase S, Bullen HE, Charnaud SC, Crabb BS, Gilson PR, de Koning-Ward TF
  in Malaria Parasites: Comparative Genomics , Evolution and Molecular Biology Ed. Carlton JM, Perkins SL, Deitsch KW. 2013 Jan; pp:199-219

2012 (6)

• Recent insights into the export of PEXEL/HTS-motif containing proteins in Plasmodium parasites.
  Bullen HE, Crabb BS, Gilson PR
  Curr Opin Microbiol. 2012 Oct; 152(6):699-704
• Plasmodium falciparum-encoded exported hsp70/hsp40 chaperone/co-chaperone complexes within the host erythrocyte.
  Kulzer S, Charnaud S, Dagan T, Riedel J, Mandal P, Pesce ER, Blatch GL, Crabb BS, Gilson PR, Przyborski JM
  Cell Microbiol. 2012 Aug; 14(11):1784-1795
• Biochemical and Functional Analysis of Two Plasmodium falciparum Blood-Stage 6-Cys Proteins: P12 and P41.
  Taechalertpaisarn T, Crosnier C, Bartholdson SJ, Hodder AN, Thompson J, Bustamante LY, Wilson DW, Sanders PR, Wright GJ, Rayner JC, Cowman AF, Gilson PR, Crabb BS
  PLoS One. 2012 Jul; 7(7):e41937
• Systematic Analysis of FKBP Inducible Degradation Domain Tagging Strategies for the Human Malaria Parasite Plasmodium falciparum.
  de Azevedo MF, Gilson PR, Gabriel HB, Simões RF, Angrisano F, Baum J, Crabb BS, Wunderlich G
  PLoS One. 2012 Jul; 7(7):e40981
• A model for the progression of receptor-ligand interactions during erythrocyte invasion by Plasmodium falciparum.
  Harvey KL, Gilson PR, Crabb BS
  Int J Parasitol. 2012 May; 42(6):567-573
• Biosynthesis, localisation and macromolecular arrangement of the Plasmodium falciparum translocon of exported proteins; PTEX.
  Bullen HE, Charnaud SC, Kalanon M, Riglar DT, Dekiwadia C, Kangwanrangsan N, Torii M, Tsuboi T, Baum J, Ralph SA, Cowman AF, de Koning-Ward TF, Crabb BS, Gilson PR
  J Biol Chem. 2012 Jan; 287(11):7871-7884

2011 (2)

• Quantifying the importance of MSP1-19 as a target of growth-inhibitory and protective antibodies against Plasmodium falciparum in humans.
  Wilson DW, Fowkes FJ, Gilson PR, Elliott SR, Tavul L, Michon P, Dabod E, Siba PM, Mueller I, Crabb BS, Beeson JG
  PLoS One. 2011 Nov; 6(11):e27705
• Toward forward genetic screens in malaria-causing parasites using the piggyBac transposon.
  Crabb BS, de Koning-Ward TF, Gilson PR
  BMC Biol. 2011 Mar; 9:21

2010 (5)

• Interactions with heparin-like molecules during erythrocyte invasion by Plasmodium falciparum merozoites.
  Boyle MJ, Richards JS, Gilson PR, Chai W, Beeson JG
  Blood. 2010 Jun; 115(22):4559-4568
• Protein kinase a dependent phosphorylation of apical membrane antigen 1 plays an important role in erythrocyte invasion by the malaria parasite.
  Leykauf K, Treeck M, Gilson PR, Nebl T, Braulke T, Cowman AF, Gilberger TW, Crabb BS
  PLoS Pathog. 2010 Jun; 6(6):e1000941
• Protein export in Plasmodium parasites: from the endoplasmic reticulum to the vacuolar export machine.
  Crabb BS, de Koning-Ward TF, Gilson PR
  Int J Parasitol. 2010 Apr; 40(5):509-513
• An aspartyl protease directs malaria effector proteins to the host cell.
  Boddey JA, Hodder AN, Günther S, Gilson PR, Patsiouras H, Kapp EA, Pearce JA, de Koning-Ward TF, Simpson RJ, Crabb BS, Cowman AF
  Nature. 2010 Feb; 463(7281):627-631
• Potential epigenetic regulatory proteins localise to distinct nuclear sub-compartments in Plasmodium falciparum.
  Volz J, Carvalho TG, Ralph SA, Gilson P, Thompson J, Tonkin CJ, Langer C, Crabb BS, Cowman AF
  Int J Parasitol. 2010 Jan; 40(1):109-121

2009 (7)

• Malaria parasites do the stick-and-slip shuffle.
  Gilson PR
  Cell Host Microbe. 2009 Dec; 6(6):499-501
• A novel family of Apicomplexan glideosome-associated proteins with an inner membrane-anchoring role.
  Bullen HE, Tonkin CJ, O'Donnell RA, Tham WH, Papenfuss AT, Gould S, Cowman AF, Crabb BS, Gilson PR
  J Biol Chem. 2009 Sep; 284(37):25353-25363
• Do apicomplexan parasite-encoded proteins act as both ligands and receptors during host cell invasion?
  Gilson PR, Crabb BS
  F1000 Biol Rep. 2009 Aug; 1:64
• A newly discovered protein export machine in malaria parasites.
  de Koning-Ward TF, Gilson PR, Boddey JA, Rug M, Smith BJ, Papenfuss AT, Sanders PR, Lundie RJ, Maier AG, Cowman AF, Crabb BS
  Nature. 2009 Jun; 459(7249):945-949
• Immunoglobulin G subclass-specific responses against Plasmodium falciparum merozoite antigens are associated with control of parasitemia and protection from symptomatic illness.
  Danielle I Stanisic, Jack S Richards, Fiona J McCallum, Pascal Michon, Christopher L King, Sonja Schoepflin, Paul R Gilson, Vincent J Murphy, Robin F Anders, Ivo Mueller, James G Beeson
  Infect Immun. 2009 Mar; 77(3):1165-1174
• Keeping it simple: an easy method for manipulating the expression levels of malaria proteins.
  de Koning-Ward TF, Gilson PR
  Trends Parasitol. 2009 Jan; 25(1):4-7
• Morphology and kinetics of the three distinct phases of red blood cell invasion by Plasmodium falciparum merozoites.
  Gilson PR, Crabb BS
  Int J Parasitol. 2009 Jan; 39(1):91-96

2008 (4)

• Comparative genomics of the neglected human malaria parasite Plasmodium vivax.
  Carlton JM, Adams JH, Silva JC, Bidwell SL, Lorenzi H, Caler E, Crabtree J, Angiuoli SV, Merino EF, Amedeo P, Cheng Q, Coulson RMR, Crabb BS, del Portillo HA, Essien K, Feldblyum TV, Fernandez-Becerra C, Gilson PR, Gueye AH, Guo X, Kang'a S, Kooij TWA, Korsinczky M, Meyer EVS, Nene V, Paulsen I, White O, Ralph SA, Ren QH, Sargeant TJ, Salzberg SL, Stoeckert CJ, Sullivan SA, Yamamoto MM, Hoffman SL, Wortman JR, Gardner MJ, Galinski MR, Barnwell JW, Fraser-Liggett CM
  Nature. 2008 Oct; 455(7214):757-763
• Inhibition of malaria parasite development by a cyclic peptide that targets the vital parasite protein SERA5.
  W Douglas Fairlie, Tim P Spurck, Joanne E McCoubrie, Paul R Gilson, Susanne K Miller, Geoffrey I McFadden, Robyn Malby, Brendan S Crabb, Anthony N Hodder
  Infect Immun. 2008 Sep; 76(9):4332-4344
• MSP1(19) miniproteins can serve as targets for invasion inhibitory antibodies in Plasmodium falciparum provided they contain the correct domains for cell surface trafficking.
  Paul R Gilson, Rebecca A O'Donnell, Thomas Nebl, Paul R Sanders, Mark E Wickham, Terry F McElwain, Tania F de Koning-Ward, Brendan S Crabb
  Mol Microbiol. 2008 Apr; 68(1):124-138
• Comparative genomics of the neglected human malaria parasite Plasmodium vivax
  Carlton JM, Adams JH, Silva JC, Bidwell SL, Lorenzi H, Caler E, Crabtree J, Angiuoli SV, Merino EF, Amedeo P, Cheng Q, Coulson RMR, Crabb BS, del Portillo HA, Essien K, Feldblyum TV, Fernandez-Becerra C, Gilson PR, Gueye AH, Guo X, Kang'a S, Kooij TWA, Korsinczky M, Meyer EVS, Nene V, Paulsen I, White O, Ralph SA, Ren QH, Sargeant TJ, Salzberg SL, Stoeckert CJ, Sullivan SA, Yamamoto MM, Hoffman SL, Wortman JR, Gardner MJ, Galinski MR, Barnwell JW, Fraser-Liggett CM
  NATURE. 2008 Jan; 455(7214):757-762

2007 (3)

• Molecular genetic tools in Toxoplasma and Plasmodium: achievements and future needs.
  Markus Meissner, Manuela S Breinich, Paul R Gilson, Brendan S Crabb
  Curr Opin Microbiol. 2007 Aug; 10(4):349-356
• Identification of protein complexes in detergent-resistant membranes of Plasmodium falciparum schizonts.
  Paul R Sanders, Greg T Cantin, Doron C Greenbaum, Paul R Gilson, Thomas Nebl, Robert L Moritz, John R 3rd Yates, Anthony N Hodder, Brendan S Crabb
  Mol Biochem Parasitol. 2007 Aug; 154(2):148-157
• A new system for rapid plasmid integration in Plasmodium parasites.
  Brendan S Crabb, Paul R Gilson
  Trends Microbiol. 2007 Jan; 15(1):3-6

2006 (1)

• Identification and stoichiometry of glycosylphosphatidylinositol-anchored membrane proteins of the human malaria parasite Plasmodium falciparum.
  Paul R Gilson, Thomas Nebl, Damjan Vukcevic, Robert L Moritz, Tobias Sargeant, Terence P Speed, Louis Schofield, Brendan S Crabb
  Mol Cell Proteomics. 2006 Jul; 5(7):1286-1299

2005 (2)

• Distinct protein classes including novel merozoite surface antigens in Raft-like membranes of Plasmodium falciparum.
  Paul R Sanders, Paul R Gilson, Greg T Cantin, Doron C Greenbaum, Thomas Nebl, Daniel J Carucci, Malcolm J McConville, Louis Schofield, Anthony N Hodder, John R 3rd Yates, Brendan S Crabb
  J Biol Chem. 2005 Dec; 280(48):40169-40176
• Tetracycline analogue-regulated transgene expression in Plasmodium falciparum blood stages using Toxoplasma gondii transactivators.
  Markus Meissner, Efrosinia Krejany, Paul R Gilson, Tania F de Koning-Ward, Dominique Soldati, Brendan S Crabb
  Proc Natl Acad Sci U S A. 2005 Feb; 102(8):2980-2985

News Articles (11)

2017 (1)

• Burnet Institute and GeoVax join forces to develop malaria vaccine

2016 (1)

• Breakthrough antimalarial study

2015 (3)

• 2015 in Review: July - December
• AMREP Prize for Burnet research
• Governor visits Burnet

2014 (2)

• Behind the Microscope Discovery Tour a great hit!
• Collaboration will advance malaria vaccine development

2013 (3)

• Eliminating malaria: vaccines, treatment and prevention.
• Congratulations to Burnet's NHMRC grant recipients
• Children help researchers solve malaria vaccine puzzle

2012 (1)

• 2012 Gust-McKenzie Medallist