Publications & Reports

Differential production of inflammatory chemokines by murine dendritic cell subsets.

Proietto AI, O'Keeffe M, Gartlan K, Wright MD, Shortman K, Wu L, Lahoud MH
The Walter and Eliza Hall Institute of Medical Research, 1G, Royal Parade, Parkville, Vic. 3050, Australia.

Abstract

Dendritic cells (DC) are efficient antigen presenting cells with the ability to activate naive T cells.

Murine DC represent a heterogeneous population that can be subdivided into distinct subsets, including the conventional DC (cDC) which are either CD4(-)CD8(-) (DN), CD4(+)CD8(-) (CD4+) or CD4(-)CD8(+) (CD8+) subsets and the plasmacytoid DC (pDC), which have different immune regulatory functions.

In this study, we investigated the differential expression of genes encoding the inflammatory chemokines Mip-1alpha, Mip-1beta and Rantes, and the secretion of these chemokines, among splenic DC subsets.

These chemokine genes were expressed at higher levels by the splenic CD4+ and DN cDC subsets compared with the CD8+ cDC, in both the resting and activated states in vivo.

Both the pDC and cDC subsets displayed increases in chemokine secretion in response to a range of toll-like receptor (TLR) stimuli in vitro.

Whilst the pDC were the highest producers of Mip-1alpha and Mip-1beta in response to some TLR stimuli, the DN and CD4+ cDC subsets were the superior producers of Rantes.

Overall, of the cDC, the CD4+ cDC produced all chemokines most efficiently, both at a basal level, and in response to most TLR stimuli.

Thus, we report a new functional difference between the murine splenic cDC subsets, with the CD4+ cDC demonstrating the most efficient production of the inflammatory chemokines Mip-1alpha, Mip-1beta and Rantes.

Publication

  • Journal: Immunobiology
  • Published: 01/01/2004
  • Volume: 209
  • Issue: 1-2
  • Pagination: 163-172