Publications & Reports

Novel sensitive real-time PCR for quantification of bacterial 16S rRNA genes in plasma of HIV-infected patients as a marker for microbial translocation.

Kramski M, Gaeguta AJ, Lichtfuss GF, Rajasuriar R, Crowe SM, French MA, Lewin SR, Center RJ, Purcell DF
Department of Microbiology and Immunology, The University of Melbourne, Parkville 3010, Victoria, Australia. [email protected]


We developed a real-time PCR to quantify 16S rRNA gene levels in plasma from HIV-infected patients as a marker of microbial translocation. The assay uses shrimp nuclease (SNuc) to eliminate DNA contamination, giving high sensitivity and low variability. The 16S rRNA gene levels measured in plasma from HIV patients correlated significantly with lipopolysaccharide levels.


  • Journal: Journal of Clinical Microbiology
  • Published: 01/10/2011
  • Volume: 49
  • Issue: 10
  • Pagination: 3691-3693


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