Publications & Reports

Immunostimulatory effects of Leishmania infantum HSP70 recombinant protein on dendritic cells in vitro and in vivo.

Manoochehr Rasouli, Mohammad Hossein Karimi, Mehdi Kalani, Salimeh Ebrahimnezhad, Manadana Namayandeh, Ali Moravej
Department of Immunology, Professor Alborzi Clinical Microbiology Research Center, Namazi Hospital, Shiraz, Iran.

Abstract

BACKGROUND: Activation of dendritic cells (DCs) has an important role in immunity against Leishmania. AIM: We investigated the effect of Leishmania infantum (L. infantum) heat shock protein 70 recombinant protein (rHSP70) as a vaccine on DC maturation and function. MATERIALS & METHODS: BALB/c mouse splenic DCs were isolated and treated with different concentrations of rHSP70. Maturation markers, cytokine production and capability of DCs to proliferate allogeneic T cells were evaluated. Furthermore, this recombinant protein was injected into BALB/c mice, and expression of CD86, CD40 and MHC class II molecules by their splenic DCs were evaluated. RESULTS: rHSP70 significantly increases the production of IL-12p70 by DCs. It had no effect on allogeneic T-cell proliferation in mixed lymphocyte reaction. It increased IFN-gamma and decreased IL-4 cytokine level in mixed lymphocyte reaction supernatant. The in vitro study showed that rHSP70 had no significant effect neither on the percentage of CD40(+), CD86(+) and MHC class II(+) DCs nor on the mean fluorescent intensity. However, in vivo results showed that rHSP70 increases the percentage of CD86-, CD40- and MHC class II-expressing cells as well as mean fluorescent intensity of CD40 and MHC class II. CONCLUSION: This study demonstrated the capability of L. infantum-derived rHSP70 in maturating BALB/c mice splenic DCs and in vivo polarization of immunity to a Th1 response.

Publication

  • Journal: Immunotherapy
  • Published: 01/05/2014
  • Volume: 6
  • Issue: 5
  • Pagination: 577-585

Author