Publications & Reports

Rapid detection of anti-Vaccinia virus neutralizing antibodies.

Kramski M, Drozd A, Lichtfuss GF, Dabrowski PW, Ellerbrok H
Robert Koch-Institute, Centre for Biological Safety, Nordufer 20, 13353 Berlin, Germany.


Increasing infections with Monkeypox and Cowpox viruses pose a continuous and growing threat to human health. The standard method for detecting poxvirus neutralizing antibodies is the plaque-reduction neutralization test that is specific but also time-consuming and laborious. Therefore, a rapid and reliable method was developed to determine neutralizing antibody titers within twelve hours. The new assay measures viral mRNA transcription as a marker for actively replicating virus after incomplete neutralization using real-time PCR.


  • Journal: Virology Journal
  • Published: 25/03/2011
  • Volume: 8
  • Pagination: 139