Publications & Reports

Ion transport blockers inhibit human rhinovirus 2 release.

Gazina EV, Harrison DN, Jefferies M, Tan H, Williams D, Anderson DA, Petrou S
Howard Florey Institute, The University of Melbourne, Vic. 3010, Australia. gazina@hfi.unimelb.edu.au

Abstract

Picornavirus replication causes leakage of cytoplasmic K+ and an influx of Na+ and Ca2+.

In this study, we have explored the possibility that a blockade of Ca2+ and Na+ influx would reduce rhinovirus production and/or release.

The Ca2+-channel blockers, verapamil and diltiazem, as well as the blocker of Na+/H+ exchange and the epithelial Na+ channel, EIPA, inhibited both virus production and release.

The effect on virus release was more pronounced than the effect on production, thus raising the possibility that rhinovirus release may serve as a target for antiviral agents.

Unexpectedly, our results also showed that the antiviral activity of the Ca2+-channel blockers was not due to the block of Ca2+ influx.

Similarly, the antiviral activity of EIPA appeared to be unrelated to the blockade of cellular Na+/H+ exchanger or the epithelial Na+ channel.

Potential alternative mechanisms of the antiviral activity of these compounds are discussed.

Publication

  • Journal: Antiviral Research
  • Published: 01/08/2005
  • Volume: 67
  • Issue: 2
  • Pagination: 98-106

Author