Publications & Reports

A central role for hepatic conventional dendritic cells in supporting Th2 responses during helminth infection.

Lundie RJ, Webb LM, Marley AK, Phythian-Adams AT, Cook PC, Jackson-Jones LH, Brown S, Maizels RM, Boon L, O'Keeffe M, MacDonald AS
Institute of Immunology and Infection Research, Centre for Immunity, Infection and Evolution, University of Edinburgh, Edinburgh, UK.

Abstract

Dendritic cells (DCs) are the key initiators of T helper (Th) 2 immune responses against the parasitic helminth Schistosoma mansoni. Although the liver is one of the main sites of Ag deposition during infection with this parasite, it is not yet clear how distinct DC subtypes in this tissue respond to S. mansoni Ags in vivo, or how the liver microenvironment might influence DC function during establishment of the Th2 response. In this study, we show that hepatic DC subsets undergo distinct activation processes in vivo following murine infection with S. mansoni. Conventional DCs (cDCs) from schistosome-infected mice up-regulated expression of the costimulatory molecule CD40 and were capable of priming naive CD4+ T cells, whereas plasmacytoid DCs (pDCs) up-regulated expression of MHC class II, CD86 and CD40 but were unable to support the expansion of either naive or effector/memory CD4+ T cells. Importantly, in vivo depletion of pDCs revealed that this subset was dispensable for either maintenance or regulation of the hepatic Th2 effector response during acute S. mansoni infection. Our data provides strong evidence that S. mansoni infection favours the establishment of an immunogenic, rather than tolerogenic, liver microenvironment that conditions cDCs to initiate and maintain Th2 immunity in the context of ongoing Ag exposure.Immunology and Cell Biology accepted article preview online, 11 December 2015. doi:10.1038/icb.2015.114.

Link to publisher’s web site

This work was supported by the Medical Research Council (MRC) UK (G0701437 to ASM) and the Wellcome Trust (LHJ). RJL is a recipient of a National Health and Medical Research Council of Australia (NHMRC) Early Career Fellowship. The authors gratefully acknowledge the contribution to this work of the Victorian Operational Infrastructure Support Program, received by the Burnet Institute.

Publication

  • Journal: Immunology and Cell Biology
  • Published: 01/02/2016
  • Volume: 94
  • Issue: 4
  • Pagination: 400-410

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