Adolescent Health in Myanmar
Support Burnet’s Adolescent Health Programs in Myanmar today.
Support Burnet’s Adolescent Health Programs in Myanmar today.
Support Burnet’s Adolescent Health Programs in Myanmar today.
Support Burnet’s Adolescent Health Programs in Myanmar today.
Background. Severe malaria (SM) is associated with high levels of cytokines such as TNF, IL-1 and IL-6. The role of chemokines is less clear, as is their cellular source.Methods. In a case-control study of children with SM (n=200), uncomplicated malaria (UM) (n=153) and healthy community controls (HC) (n=162) in Papua New Guinea, we measured cytokine/chemokine production by Peripheral Blood Mononuclear Cells (PBMCs) stimulated with live P. falciparum parasitised red blood cells (pRBC). Cellular sources were determined. Associations between immunological endpoints and clinical/parasitological variables were tested.Results. Compared to HC and UM, children with SM produced significantly higher IL-10, IP-10, MIP-1beta and MCP-2. TNF and MIP-1alpha were significantly higher in the SM compared to the UM group. IL-10, IL-6, MIP-1alpha, MIP-1beta and MCP-2 were associated with increased odds of SM. SM syndromes were associated with distinct cytokine/chemokine response profiles compared to UM cases. TNF, MIP-1beta and MIP-1alpha were produced predominantly by monocytes and gammadelta T cells, and IL-10 by CD4+ T cells.Conclusions. Early/innate PBMC responses to pRBC in vitro are informative as to cytokines/chemokines associated with SM. Predominant cellular sources are monocytes and gammadelta T cells. Monocyte-derived chemokines support a role for monocyte infiltrates in the aetiology of SM.
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Publisher’s final pdf available at http://jid.oxfordjournals.org/content/210/2/295.long