Publications & Reports

Cloning and expression of a second human natural killer cell granule tryptase, HNK-Tryp-2/granzyme 3.

Sayers TJ, Lloyd AR, McVicar DW, O'Connor MD, Kelly JM, Carter CR, Wiltrout TA, Wiltrout RH, Smyth MJ
Biological Carcinogenesis and Developmental Program, SAIC Frederick, National Cancer Institute, Frederick Cancer Research and Developmental Center, Maryland 21702, USA.


Cytotoxic lymphocytes possess a number of serine proteases (granzymes) usually localized in cytoplasmic granules. To date, the DNA sequences of four human granzymes have been reported. A fifth human granzyme (granzyme 3) has been biochemically purified and its N-terminal amino acid sequence has been reported. This enzyme was described as possessing tryptase activity, cleaving synthetic substrates after arginine or lysine. We recently cloned a rat granzyme tryptase (RNK-Tryp-2), and used this cDNA to screen human cDNA libraries. Isolation of cDNA fragments of a human gene could be overlapped to provide a complete cDNA sequence, which we designated HNK-Tryp-2. The N-terminal amino acid sequence deduced from HNK-Tryp-2 was identical to that reported for granzyme 3. This gene appears to be a single copy gene that is expressed in isolated natural killer cells and T cells as well as in tissues containing these cells.


  • Journal: Journal of Leukocyte Biology
  • Published: 01/05/1996
  • Volume: 59
  • Issue: 5
  • Pagination: 763-768