Publications & Reports

Isolation and characterization of cDNA clones for Humly9: the human homologue of mouse Ly9.

Sandrin MS, Henning MM, Lo MF, Baker E, Sutherland GR, McKenzie IF
Molecular Immunogenetics Laboratory, Austin Research Institute, Austin Hospital, Heidelberg, Vic, Australia.

Abstract

Ly9 is a mouse cell membrane antigen found on all lymphocytes and coded for by a gene that maps to chromosome 1. We previously described the isolation and characterization of a full-length cDNA clone for mouse Ly9. Using cross-species hybridization we isolated cDNA clones encoding the human homologue Humly9. Analysis of the predicted protein sequence suggests that the extra-cellular portion of the Humly9 molecules is composed of four Ig-like domains: a V domain (V) without disulphide bonds and a truncated C2 domain (tC2) with two disulphide bonds, a second V domain without disulphide bonds and a second tC2 with two disulphide bonds, i.e., as V-tC2-V-tC2. The gene encoding Humly9 was mapped to chromosome 1 by analysis of human/hamster hybrids, and more specifically to the 1q22 region by in situ hybridization. The protein sequence data support the view that Humly9 belongs to the immunoglobulin-superfamily subgroup which includes CD48, CD2, and LFA-3.

Publication

  • Journal: Immunogenetics
  • Published: 01/03/1996
  • Volume: 43
  • Issue: 1-2
  • Pagination: 13-19