Publications & Reports

Hepadnavirus envelope topology: insertion of a loop region in the membrane and role of S in L protein translocation.

Grgacic EV, Kuhn C, Schaller H
Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia. [email protected]


A unique feature of the large hepadnavirus envelope protein (L) is its mixed transmembrane topology, resulting from partial posttranslational translocation of the pre-S domain. Using protease protection analysis, we demonstrate for duck hepatitis B virus an essential role for the small envelope protein (S) in this process, providing the first experimental evidence for an S translocation channel. Further analysis revealed that the presumed cytoplasmic loop between TM1 and TM2 in the C-terminal S domain is membrane embedded and protrudes to the particle surface. These data suggest that some L molecules form a highly folded, potentially spring-loaded topology with five membrane-spanning regions and a membrane-traversing pre-S chain.


  • Journal: Journal of Virology
  • Published: 01/03/2000
  • Volume: 74
  • Issue: 5
  • Pagination: 2455-2458