Projects

The role of monocytes in the development of cardiovascular disease

Cardiovascular disease (CVD) is a leading cause of death world-wide, and certain populations who experience chronic inflammation, including aged individuals and people living with HIV (PLWH), have an increased risk of CVD. Most CVD starts with a thickening of the artery wall known as atherosclerosis.

We have developed a unique in vitro model of early atherosclerotic events that allows us to study the process of blood monocytes migrating into the blood vessel wall and forming fat-filled cells called “foam cells” which drive atherosclerosis. Using this assay, we have made the novel discovery that monocytes from aged individuals and PLWH have a greater propensity to develop into these atherosclerosis-promoting foam cells, and that this process is heightened by inflammation. What causes this is not known, but we are exploring the following:

  • Investigating a novel species of “good cholesterol” found in PLWH which is dysfunctional and heightens foam cell formation.
  • Isolating mRNA from monocytes from aged individuals and PLWH and using RNASeq to analyse the expression of genes involved in cholesterol synthesis, metabolism and transport.
  • Assessing cholesterol uptake and efflux by monocytes using established assays.
  • Exploring treatments and drugs which reverse or inhibit cholesterol accumulation and foam cell formation.

Timeline

2016 – 2019

Collaborators

Associate Professor Anthony Jaworowski, RMIT University Dr Tom Angelovich, RMIT University

Contact Details

For any general enquiries relating to this project, please contact:

Doctor Anna Hearps

Deputy Program Director, Healthy Ageing (Expansion Program)

Email

anna.hearps@burnet.edu.au