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Visualisation of phenotypically mixed HIV-1 and HTLV-I virus particles by electron microscopy.

Lawson VA, Lee JY, Doultree JC, Marshall JA, McPhee DA

  • Journal Journal of biomedical science

  • Published 08 Mar 2000

  • Volume 7

  • ISSUE 1

  • Pagination 71-4

  • DOI 10.1007/BF02255921


Virions produced after HIV-1 infection of HTLV-I transformed cells have an expanded tropism that has been attributed to the presence of HTLV-I glycoproteins in the envelope. This report now directly identifies these phenotypically mixed virions by immunogold labelling electron microscopy. Furthermore we estimate there are 2% of these in cell-free supernatant, which represents up to 1 x 10(7) particles/ml from an in vitro infection. HTLV-1 envelope labelling was localised to a single region, suggesting a defined event in packaging of foreign envelope proteins into HIV-1 virus particles.