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The immune response of mice and cynomolgus monkeys to macaque mucin 1-mannan.

Vaughan HA, Ho DW, Karanikas V, Sandrin MS, McKenzie IF, Pietersz GA

  • Journal Vaccine

  • Published 07 Sep 2000

  • Volume 18

  • ISSUE 28

  • Pagination 3297-309

  • DOI 10.1016/s0264-410x(00)00143-2


Mice immunised with human epithelial mucin MUC1 coupled to oxidised mannan produce MUC1 specific MHC Class 1 restricted CD8(+) cytotoxic T cells and are completely protected from the development of MUC1(+) tumours; such therapy may be applicable to humans. In this light we describe pre-clinical studies in cynomolgus monkeys (Macaca fascicularis), to test the efficacy of mannan-MUC1 in higher primates. Monkey MUC1 genomic clones were isolated from a macaque library, peptides and fusion protein synthesised and mice and monkeys immunised with macaque MUC1-mannan. In mice CTL responses were induced (as has been found with human MUC1 mannan conjugates), but in contrast monkeys produced a humoral response, with no T cell proliferative, cytotoxic responses or CTLp found. In spite of the presence of anti-MUC1 auto-antibodies, there was no toxicity or induction of autoimmunity.