close Icon

Hepatitis A virus replication: an intermediate in the uncoating process.

Bishop NE

  • Journal Intervirology

  • Published 21 Jun 2000

  • Volume 43

  • ISSUE 1

  • Pagination 36-47

  • DOI 10.1159/000025021


Dense, RNase-sensitive, VP2-containing, non-infectious hepatitis A virus (HAV) particles were found to be formed at early times after the infection of cultured cells. These particles formed with kinetics mirroring those reported for HAV uncoating. The kinetics of the formation of dense HAV particles corresponded to a decrease in detectable, mature input virions, as detected by RNA dot blot hybridization of CsCl density gradient fractions. The dense HAV particles did not appear to have altered sedimentation coefficients, and as the fate of small capsid protein VP4 is not yet known, these particles cannot yet be termed 'A particles' or 'infectosomes', as have the uncoating intermediates in some picornavirus-cell systems.