Burnet Cell Imaging Facility

Assists scientists at all levels and from all disciplines to visualise and track the movement the cells.

Burnet’s Cell Imaging Facility (CIF) provides guidance on the use of the facility especially in the areas of project design, training and support in image acquisition and analysis.

As part of the Monash Micro Imaging (MMI) network, the CIF gains access to extensive expertise and imaging platforms located on Monash University Clayton campus and the Monash Health and Research Precinct.

CIF services are available to all researchers at the Alfred Medical Research and Education Precinct (AMREP) as well as external users.

Scientific staff from Melbourne’s leading research centres use CIF services to acquire images on a diverse range of diseases including malaria, HIV and AIDS, influenza, hepatitis C, heart disease, diabetes and cancer.

Equipment

Nikon N-SIM / N-STORM super-resolution microscope

This state-of-the-art fluorescence microscope combines the N-SIM and N-STORM technologies to achieve the best possible resolution.

Features of this microscope are:

  • Motorized inverted Nikon ECLIPSE Ti-E microscope
  • Perfect focus system, motorized XY stage with encoders and piezo Z stage
  • N-SIM Lasers: Sapphire 488-200 and 561-150
  • N-STORM: Sapphire 488-100 and 561-100 and OBIS 405 and 647
  • Imaging modes: TIRF-SIM, 2D-SIM, 3D-SIM, TIRF-STORM and 3D-STORM
  • Andor Technology iXon DU897 EMCCD camera
  • NIS-Elements software
  • Objectives: 10X (0.45), 60x (1.27) and 100X (1.49)
  • Resolution N-SIM: 100nm (XY axis) and 300nm (Z axis)
  • Resolution N-STORM: 20nm (XY axis) and 50nm (Z axis)

Located in the BSL2 Containment Laboratory.

API DeltaVision Deconvolution Microscope

This is the only microscope in Australia that can be used to image live cells infected with highly infectious human pathogens such as the human immunodeficiency virus (HIV).

Features of this microscope are:

  • Deconvolution software
  • Widefield inverted microscope
  • Motorized excitation and emission filter wheels
  • Excitation with a mercury arc lamp
  • Four channel fluorescence imaging
  • Cascade 512 EMCCD and CoolSNAP CCD cameras
  • Motorized XYZ stage suitable for ‘stitching’ as well as ‘mark and find’ experiments
  • Three photo-ablation/activation lasers (410nm, 488nm and 532nm)
  • 10x, 20x, 40x oil, 60x oil, 100x oil objectives
  • Incubator (gas, temperature and humidity control) for live cell imaging

Located in the BSL3 Containment Laboratory

Zeiss Cell Observer

Features of this microscope are:

  • Widefield motorized inverted microscope
  • Excitation with metal halide lamp or LED
  • Four channel fluorescence imaging
  • DIC and Phase Contrast
  • AxioCam MRm and AxioCam HSm CCD cameras
  • Motorized XYZ stage with ‘definite focus’ suitable for ‘stitching’ as well as ‘mark and find’ experiments
  • 10x, 20x, 40x, 63x oil, 100x oil objectives
  • Incubator (gas, temperature and humidity control) for live cell imaging
  • Deconvolution software

Located in the BSL2 Containment Laboratory.

Olympus IX51

Features of this microscope are:

  • Widefield manual inverted microscope
  • Excitation with a mercury arc lamp
  • Four channel fluorescence imaging
  • DIC and Phase Contrast
  • XM10 CCD camera
  • 4x, 10x, 20x, 40x, 100x oil objectives

Located in the BSL2 Containment Laboratory.

Access

Conditions of use:

Every researcher is given a tutorial before being permitted to use a microscope. Please contact us prior to the start of an imaging project (appointments are essential).

The BCIF should be informed of any publication and/or presentation referring to the BCIF.

Equipment booking:

MMI Calcium Calendar

Click on the ‘Make A Booking tab’

Contact Details

For any general enquiries relating to this facility, please contact:

Doctor Paul Gilson

Group Head, and Head of Burnet Cell Imaging Facility

Telephone

+61385062481

Email

paul.gilson@burnet.edu.au